Has OriGene fully sequenced all TrueORF clones?
Not always. When transferring the cDNA into the TrueORF Entry Vector, OriGene always uses fully sequenced plasmids as templates and Phusion High-Fi¬delity DNA Polymerase (New England Biolabs), which has a mutation rate less than 4 x 10-7. This ensures the highest fidelity of every TrueORF clone. After cloning into the Entry vector, each of OriGene’s TrueORF clones was sequenced at both the 5’ and 3’ ends, and the resulting sequence was matched to the corresponding reference sequence. For ORFs of 1 Kb or less in length, the 5’ and 3’ sequencing reads have covered the full ORF. For longer cDNAs, the ORF was not fully covered by sequencing reads.
