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For recovery of the immunoprecipitated DNA, can I get better results with a phenol chloroform extraction instead of a column extraction?

April 26, 2017chloroform column dna extraction immunoprecipitated phenol recovery results

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For recovery of the immunoprecipitated DNA, can I get better results with a phenol chloroform extraction instead of a column extraction?

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The efficiency and yield of the DNA extraction is typically higher using a phenol chloroform extraction. However, same people still use column extraction to get the DNA for downstream applications such as PCR or T7 amplification for microarray (ChIP-chip) analysis. Also on this way our kit was validated for microarray analysis. Pooling multiple samples prior to DNA purification may improve yields for column extractions.