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For quantitative real-time PCR applications, what is the optimal distance from the 3-prime poly (A) tail for design of primer/probe sets?

April 26, 2017applications design distance optimal PCR Poly quantitative real-time tail

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For quantitative real-time PCR applications, what is the optimal distance from the 3-prime poly (A) tail for design of primer/probe sets?

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Due to the amplification mechanism of the Applause 3′-Amp System, we recommend primer/probe sets be designed within the first 1,500 bases from the poly (A) tail.