For proteins secreted into the medium, is the presence of free biotin in the medium a problem (eukaryotic expression)?
The amount of biotin depends on the cell line and the medium. Some media for insect cells or mammalian cells contain up to 800 nmol biotin per litre and has to be removed or blocked before applying the lysate to Strep•Tactin columns. For mammalian cell culture only DMEM and Leibovitz’s L-15 media are free of biotin. For insect cell culture, only Schneider’s medium is free of biotin. Serum may also contain biotin. However, serum tested (FCS, PAA) did not contain measurable amounts of biotin (<0.025 mg/ml; <0.1 mM). Ingredients of proprietary formulations for serum free growth are usually not disclosed, but information on biotin content can be obtained and should be requested from the respective manufacturer.
Related Questions
- Is the presence of free biotin in the medium (in case of proteins secreted to the medium) a problem (eukaryotic expression)?
- Is the presence of biotinylated proteins in the host organism in case of proteins expressed in the cytoplasm a problem?
- For proteins expressed in the cytoplasm, is the presence of biotinylated proteins in the host organism a problem?
