For in vitro use (cell culture studies), is CsCl- or chromatography purification required?
No. If viruses will be used in in vitro cell cultures, double CsCl purification is not required. For in vivo studies (i.e. animal studies), purification is essential in order to remove defective particles, cell debris, and small amounts of media components, since these contaminants induce significant immune responses. In addition, CsCl purification will concentrate the virus to a level suitable for in vivo injections. Q. What are the recommended storage conditions of recombinant Adenoviruses? For long-term storage, the virus should be kept at -80°C, especially after CsCl or chromatography purification. At -80°C, the virus could be stable for 6 months to a year and in some cases even up to 2 years. If the virus is in DMEM supplemented with serum or BSA and stored at -80°C, it will remain stable for longer periods of time and through several freeze-thaw cycles. On the other hand, if the virus CsCl-purified and kept in PBS or Tris solution (20mM Tris PH8.0, 200 NaCl, 2-3% glycerol or sucr
