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For detecting topo/DNA cleavages using drug screening kits, how much enzyme is required?

April 26, 2017detecting dna drug enzyme Kits screening topo

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For detecting topo/DNA cleavages using drug screening kits, how much enzyme is required?

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Usually these assays require 2 ul of undiluted enzyme; in other words more than 4 units of activity. In some cases you may be able to use 1 ul, but this depends upon the actual lot of enzyme being tested; as noted above, some lots are more active than others.