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Doesn the residual fat emulsion in the QuickTest cloud the TSB media and make seeing microbial-caused turbidity difficult?

April 26, 2017Cloud emulsion fat media QuickTest residual seeing tsb

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Doesn the residual fat emulsion in the QuickTest cloud the TSB media and make seeing microbial-caused turbidity difficult?

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Residual fat emulsion in the filter chamber would be a problem if we didn’t have an effective two-step procedure for removing it. The bag of GroMed™ media that comes with each QuickTest contains approximately 20cc of air and 100mL of TSB. After filtering the admixture, the operator can perform an “air purge” of the filter chamber with the sterile air. This eliminates most of the residual fat emulsion. Then the filter is rinsed with 40 to 80mL of the sterile TSB. The last 20mL of TSB is left in the filter chamber to grow potential microbial contaminates.