Does your lab provide bisulfite conversion for methlyation studies?
No. Each investigator must carry out their own bisulfite conversion. At this time, Illumina recommends the use of the Zymo EZ-DNA Methylation kit for the conversion process Bisulfited DNA is single stranded and unstable, so please be sure to arrange a slot in the SNP Center lab with your project manager before beginning the process. • What is the difference between Golden Gate and Infinium chemistry? For detailed information on the different chemistries, please see Illumina’s website. A description of the Golden Gate Chemistry is here. A description of the Infinium Chemistry is here. • If I don’t have any family structure in my samples, do I need to provide a pedigree file? Yes. Our computer scripts require a pedigree file to function. Files without family structure are generally very easy to create following the instructions provided in your initioation documents. If you have misplaced the directions, please contact your project manager.
