Does total RNA have to be isolated beforehand?
No. The µMACS™ Technology allows high-quality mRNA isolation directly from various biological materials. The two main reasons are: i) The extremely small (diameter 50 nm), paramagnetic MicroBeads enable an efficient labeling of mRNA within seconds; while, ii) MACS® Column Technology simplifies the required washing steps. If a two-step mRNA isolation from total RNA is favored, µMACS mRNA isolation is the method of choice to obtain high-quality mRNA.
