Does this Two-Cycle cDNA Synthesis Kit/protocol work well on partially degraded RNA samples?
No. Similar to previous recommendations for the standard protocol and SSTLvII, the Two-Cycle cDNA Synthesis Kit protocol also requires high-quality total RNA as starting material. Most commonly, customers assess OD260/280 ratios together with electrophoretic analysis results when sufficient material is available. When sample quantity is limiting, the combination of RiboGreen for quantitation and Bioanalyzer for quality assessment has worked quite well. To better distinguish whether the starting material quality has affected the target labeling process, it is recommended to use the GeneChip® Eukaryotic Poly-A RNA Control Kit and spike the positive controls into the starting materials for efficient troubleshooting.
