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Does this replace the use of antibodies for Western blot analysis or qRT-PCR knockdown detection?

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Does this replace the use of antibodies for Western blot analysis or qRT-PCR knockdown detection?

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No. This system is recommended as a prescreening method to choose the most effective construct with which to do your experiments. It is still advised to perform Western blot analysis, qPCR, or functional assays to monitor the level of gene knockdown.

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