Does this method detect methylation at specific CpG dinulceotides?
No. This method examines the methylation status across a CpG-rich sequence, which correlates to biological phenotypes much better than the methylation status of any individual CpG dinulceotides. Other methods only analyze one or two CpG sites in one assay and assume that their methylation status represents the status of the surrounding target sequence. These methods include Southern blot, MSRE and RLGS, and bisulfite conversion-based methods like MSP, TaqMan-based MethyLight, MS-SNuPE, GoldenGate DNA Methylation BeadArray, Infinium DNA Methylation BeadChip, and COBRA.
