Does this assay detect HIV-1 gp120 of isolates from all clades?
This assay was designed for detection of clade B viruses. Due to the extensive gp120 sequence variability among clades, binding of non-B gp120 will, generally, occur with lower affinity. As such, sensitivity is greatly reduced, making the calculation of the gp120 concentration, based on the supplied standard, impossible. However, it would be possible to quantify the amount of any reactive gp120 if a standard is prepared from the same isolate.
