Does the SPERM HY-LITER staining protocol damage sperm or epithelial cells?
The staining procedure is simple, and extremely reproducible and works particularly well on highly dense cell preparations. Cells are not damaged in any way, and no morphology, if it is originally present, is lost during the staining method. If tails, midsection and acrosomes are present at the beginning of the staining, they will be present at the end – however the antibody targets the sperm head, the sole remaining sperm substructure in sexual assault evidence, uniquely.
