Does the same U1 promoter in the SureSilencing™ shRNA plasmids express shRNA in human, mouse, and rat cell lines?
Yes, the human, mouse, and rat Pol II enzymes transcribe from the human U1 promoter of the SureSilencing™ shRNA Plasmids with equal efficiency and effectiveness. The sequence identity of the promoters in the three different species is similar enough that enzymes from each species will recognize and bind the others.
Related Questions
- How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
- Does the same U1 promoter in the SureSilencing™ shRNA plasmids express shRNA in human, mouse, and rat cell lines?
- What cell line does SABiosciences use for quality control testing of the SureSilencing™ shRNA plasmids?
