Does the RampUP procedure amplify ribosomal RNA and if so, what are the consequences?
Yes, ribosomal RNA will be amplified by the RampUP process, however, the consequences are negligible when dealt with properly. For microarray analysis, it is critical to maintain appropriate stringency to minimize the impact of ribosomal RNA to the array data. In addition, the presence of ribosomal RNA in the amplified product will require an increase in the amount of senseRNA for downstream analysis (typically by a factor of about five over amplification products generated with a dT primer alone).
