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Does the NCode SYBR Green miRNA qPCR kit require isolation of miRNA for detection and quantification of miRNA ?

April 26, 2017detection isolation kit mirna qPCR Quantification require

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Does the NCode SYBR Green miRNA qPCR kit require isolation of miRNA for detection and quantification of miRNA ?

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No, miRNA isolation is not required. These kits have been optimized for the detection of miRNA from 10 ng to 2.5 μg of total RNA using a miRNA specific forward primer and an universal qPCR primer.