Does the frequent fill mode affect the diffraction images?
Under normal data collection conditions, we cannot detect any substantial differences between the quality of the data collected in frequent fill mode and with constant stored beam. It is also impossible to determine whether an injection took place during the collection of a diffraction image by examining the reflections in that image. Note that the frequent fill mode eliminates to a great extent the need to correct the exposure time for the images over the data collections. See ”When to use exposure control”. If a manual injection takes place, the data collection software will stop and restart when the temperature of the beamline optics has stabilized. If an image was being collected during manual injection, it will be recollected.