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Does the DNA methylase Eco dam pair nucleotide sequences to form site-specific duplexes?

April 26, 2017Dam dna duplexes eco form nucleotide pair sequences site-specific

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Does the DNA methylase Eco dam pair nucleotide sequences to form site-specific duplexes?

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The Eco dam methylase is active on denatured DNA and single-stranded synthetic oligonucleotides containing GATC sites. The results suggest that on interaction with single-stranded oligonucleotides the Eco dam methylase is able to form a duplex structure within the GATC site, and that this duplex site is a substrate for enzyme.