Does the compound or analyte have an effect on transfer?
In general, there is little or no effect on transfer volumes when the concentration of the dissolved compound is between 4 and 8 mg/mL (or 10 mM for compounds with MW between 400 and 800). This poster “Impact of Compound Concentration” shows that accuracy was affected by less than 2% and precision by less than 3% even when the solubility of the compound forced the material into a suspension. If precipitate collects at the bottom of the well and occludes the acoustic energy, the Echo system will skip the transfer from that particular well and record the anomaly. Back to main list.
