Does the Bacillus circulans CBD have an affinity for low molecular weight chitin (2 to 6 GlcNAc residues)?
No, two different tests have been performed which illustrate that the CBD does not bind to these low molecular weight chitin molecules. One was to try eluting the CBD fusions bound to chitin with low MW sugars of chitin, 1 to 6 GlcNAc residues. The fusion remained bound to the chitin when washed with each of these six different length chitin sugars, showing that at least the CBD has a higher affinity for the high MW chitin. In another test CBD fusions were loaded onto columns that had single GlcNAc residues or triacetylchitotriose residues linked to agarose beads. No binding was detected in either case.
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- Does the Bacillus circulans CBD have an affinity for low molecular weight chitin (2 to 6 GlcNAc residues)?
