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Does the amount of plasmid DNA used in the transfection effect the performance of the GeneSwitch® system?

April 26, 2017dna PERFORMANCE plasmid system transfection Used

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Does the amount of plasmid DNA used in the transfection effect the performance of the GeneSwitch® system?

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We have found that overexpression of the GeneSwitch protein increases basal expression, which lowers fold induction levels. Therefore, we recommend using no more than 300 ng of each plasmid when transfecting 1-5 x 105 cells.