Does TAP Express completely replace traditional cloning of genes?
TAP Express replaces traditional cloning at the stage of functional screening. This is particularly advantageous when a large numbers of genes are to be analyzed. For example, using traditional cloning the time and labor necessary to analyze 30 genes would take months. However, with TAP Express system, screening this many genes is now possible within a few days. Of course, once gene(s) with the desired function have been identified, cloning would be necessary to verify the gene sequences and to do further gene structure/function analysis studies. Towards these latter applications, Genlantis has made available the TAP Express Cloning Kit (Cat. # TAPC2010) an enzyme-free cloning system that allows for easy and rapid cloning of any TAP-generated fragment.
