Does secondary structure formation occur with peptides that are synthesized on the membrane?
Yes. Secondary structures in peptides can form in sequences as short as 6 amino acids. However, the secondary structure formed by a peptide on a SPOTs membrane may not necessarily resemble the secondary structure formed by the same peptide within the full length native protein. Hence, epitope mapping results should be interpreted with the caveat that the sequence recognized by the antibody may form a conformational epitope.
