Does R&D Systems offer a positive control for the Caspase Activity Assays?
R&D Systems offers various recombinant Caspase enzymes and they are ideal positive controls. If one prefers to generate a positive control, there are several published methods to generate apoptotic positive control samples. These include incubating cells with 2 micromolar Staurosporin for 2 hours. This induces apoptosis in MOST cell types. R&D Systems’ Technical Service also has a list of references for generating positive controls for specific Caspases.