Does PPS Silent Surfactant stick to reverse phase column material?
The hydrophobic portion of cleaved PPS Silent Surfactant is 1-hexanol, which does not associate strongly with any kind of reverse phase material. Unexpected downstream effects may be caused by the presence of uncleaved surfactant. In this case, cleavage conditions should be optimized by reducing the pH of the digest buffer by one unit or by extending the reaction time interval ten-fold.
