Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Does Phusion® Hot Start DNA Polymerase need a separate activation step in the PCR protocol?

April 26, 2017activation dna hot PCR phusion® polymerase Protocol separate step

0

Posted

Does Phusion® Hot Start DNA Polymerase need a separate activation step in the PCR protocol?

1 Answer

0

Posted

No. Phusion® Hot Start DNA Polymerase is inactivated by a reversibly bound, specific Affibody® protein, which releases the polymerase fully active at polymerization temperature.