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Does Hypoxyprobe -1 penetrate hypoxic brain and brain tumor tissue?

April 26, 2017brain hypoxic hypoxyprobe PENETRATE tissue tumor

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Does Hypoxyprobe -1 penetrate hypoxic brain and brain tumor tissue?

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Although Hypoxyprobe -1 is water soluble, its corresponding free base has an octanol water coefficient of 8.5 and, as a result, the marker freely penetrates into both brain and brain tumor tissue (48, 49). Photomicrograph of a E106 human gliomablastoma multiforme xenograft growing in the brain of a nude mouse. click to see full size image(244Kb) Immunofluorescence staining of the frozen section reveals vasculature (ME 9F1; red) and hypoxia (Hypoxyprobe -1 adducts; green) in combination with perfusion (Hoechst 33342;(blue) in normal brain (N) and tumor tissue (T). Original magnification x 200. Note the regular pattern of vasculature in normal brain versus poorly organized vasculature in tumor tissue (Bernsen et al, Journal of Neurosurgery 93: 449-454, 2000; by permission)(43).