Does glutamine act as a substrate for transamination reactions in the liver of fed and fasted sheep?
The present study investigated the relative importance of glutamine as a transamination source in the ovine liver by examination of the labelling of amino acids (AA) in the hepatic free pool, mixed liver and plasma proteins of fed and fasted sheep, following infusion of isotopically-labelled glutamine. In a cross-over design four sheep were either fasted for 3 d or fed to maintenance and finally euthanased. At each intake, the sheep were infused for 6 h with [2-15N]glutamine (150 micromol/h) and samples of total plasma protein isolated. Following the terminal infusion, liver tissue total proteins were prepared and hydrolysed and 15N-enrichments in seventeen AA were determined by GC-combustion-isotope-ratio mass spectrometry. All AA were enriched (relative to natural abundance) except lysine and threonine, with the lowest enrichments in phenylalanine and histidine. There was no effect of the fed v. fasted state, except for leucine and isoleucine in liver protein Enrichments in liver pro
