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Does freezing and thawing decrease activity of the reagents?

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Does freezing and thawing decrease activity of the reagents?

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A8: Before reconstitution freezing and thawing does not affect the reagents. Reconstituted reagents containing luciferin can be frozen at -80°C but not at -20°C. Repeated freezing and thawing should be avoided. All other reagents and diluents including ATP standards can be frozen and thawed.

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