Does Covalab prepare phosphospecific antibodies?
Covalab produces phosphospecific antibodies and our method follows the procedure below: The phosphorylated peptide will be conjugated and used to immunise the host animals. Following the final bleed, phosphospecific antibodies will be selected by affinity purification. Two affinity columns will be used, the first one with non-phosphorylated peptide and the second one with the phosphorylated peptide. The serum will be passed through the first column and the flow –through will be kept. The flow –through will be purified on the second column in order to isolate those antibodies which recognise the phosphorylated peptide. ELISA tests will be performed to ensure that the antibodies recovered from the second column are phosphospecific.
