Does bacterial host strains have impact for DNA sequencing quality?
DH5 alpha host strains consistently produce good results. HB101, MV1190, JM109 and XL1 Blue host strains show some variability in result quality. XL1 Blue grows slower than most strains and can lead to decreased DNA yields and it does not respond to TB as do other strains (only showing a 2-3 fold increase in cell number per ml). JM101 is not recommended.
