Does AquaGenomic Solution contain Proteinase K?
No. AquaGenomic can be used to extract DNA from cells and tissues without needing protease digestion. However, if mitochondrial DNA (mtDNA) recovery is desired, you will need to add Proteinase K to AquaGenomic solution to 100 mg/ml and incubate the sample at 60-65 C for 1-2 hours during cell lysis and then at 95 for 10 minutes to inactivate the Proteinase K.
No. AquaGenomic can be used to extract DNA from cells and tissues without needing protease digestion. However, if mitochondrial DNA (mtDNA) recovery is desired, you will need to add Proteinase K to AquaGenomic solution to 100 mg/ml and incubate the sample at 60-65° C for 1-2 hours during cell lysis and then at 95 for 10 minutes to inactivate the Proteinase K.
No. AquaGenomic can be used to extract DNA from cells and tissues without needing protease digestion. However, if mitochondrial DNA recovery is desired, you will need to add Proteinase K to AquaGenomic solution to 100 mg/ml and incubate the sample at 60-65° C for 1-2 hours during cell lysis and then at 95 for 10 minutes to inactivate the Proteinase K.
No. AquaGenomic can be used to extract DNA from most cells and tissues without needing protease digestion. However, for DNA extraction from dry blood spot, saliva, and some fibrous tissues, or if mitochondrial DNA recovery is desired, you will need to add Proteinase K to AquaGenomic solution to 50 ug/ml (e.g., add 1 ul of 5 mg/ml Proteinase K stock to 100 ul of AquaGenomic solution) and incubate the samples at 60 °C for 60 min and then at 95 for 10 min to inactivate the Proteinase K.
