Does Applied Biosystems have a document that contains chemistry optimization guidelines for the ABI PRISM® 7000, ABI PRISM® 7900HT?
Yes, Applied Biosystems has developed TaqMan® Quick Assay Development Guidelines for the following applications: DNA and RNA quantitation, and Allelic Discrimination. The Quick Assay Development Guidelines are published in the TaqMan® Universal PCR Master Mix protocol booklet (P/N 4304449). The Universal PCR Master Mix protocol can be found here. You can also find this information by downloading the Sequence Detection Systems Quantitative Assay Design and Optimization guide from our documentation library.
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