Does AmpliTaq Gold™ leave an A overhang as does AmpliTaq® DNA Polymerase?
AmpliTaq Gold™ is the same enyzme as AmpliTaq® DNAPolymerase except that it is supplied in the inactive state. Once activated it has the same properties and characteristics as AmpliTaq. Therefore, since it does not have “proofreading” ability, it too will leave an A overhang. This happens arbitrarily, and as with AmpliTaq DNA Polymerase, in order to drive the reaction to the extra A state, the final extension time at 72°C should be increased to 15-30 minutes.
