Do you recommend the use of a high glucose containing medium in the CELLine?
Most protocols were developed using standard RPMI-1640 medium. Some customers and others who use hollow fiber bioreactors do use richer mediums. A slight performance increase may be obtained with richer media, however, this is dependent upon cell line and should be evaluated experimentally. In general, excellent results are obtained in medium that is currently used to culture the cell line in static flasks.