Do you provide synthetic siRNA oligos?
No. Genscript does not provide synthetic siRNA oligos. However, GenScript vector-based siRNA or siRNA cassette can function the same way as synthetic oligos. • Will you guarantee that the recommended siRNA constructs work? A: By using GenScript siRNA design center and custom siRNA, there is 95% chance that you will find a potent siRNA target (70% inhibition) if you test 3 siRNA constructs for a gene. The most common problem for siRNA failure is the transfection efficiency. GenScript has developed GFP marker vector to address that issue. However, Genscript does not guarantee that the recommended siRNA constructs will work because of uncertainty in the transfection experiments. • How many constructs do you recommend to order for a gene? A: We recommend that at least 3 siRNA constructs should be tested for each gene. • Do you have a protocol for transfection using siRNA constructs? A: Yes. You can download the protocol using this link. • What kind of transfection reagents should I use for
