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Do you have a protocol or reference for the researcher to show him HOW to use Heparan Sulfate (#400700) as a positive control for digestion?

April 26, 2017CONTROL digestion positive Protocol Reference researcher Sulfate

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Do you have a protocol or reference for the researcher to show him HOW to use Heparan Sulfate (#400700) as a positive control for digestion?

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You can dissolve 1 mg of HS (400700) in 100 ml of 100mM sodium acetate buffer pH 7.0 containing 10mM calcium acetate and add 10mU of Heparitinase which is dissolved in the same buffer (100?l/vial). You should incubate the mix solution at 37oC for 10 hours.