Do you have a method for coating the PVP-Free Polycarbonate membranes for Chemotaxis?
A. General Chemotaxis Notes: Membranes are used in the study of white blood cell reactions to toxins (chemotaxis), to determine the natural immunity in whole blood. Since immunity is transferable, this could lead to the development of vaccines for treatment of cancer and other diseases. Membranes may be polycarbonate, with or without polyvinylpyrrolidone (PVP). It depends on the type of cells used. Apparently, Neutrophils have a tendency to round up and fall off the membrane when around PVP – not so with some of the other types of cells. Refer to each procedure.