Do trace elements modify the activity of erythrocyte sodium-proton exchanger in hemodialyzed patients?
The kinetics (Vmax and Km) of the erythrocyte Na+-H+ exchanger was studied in a group of 21 patients undergoing regular hemodialysis (HD) and in 21 control subjects. The activity of antioxidative enzymes–superoxide dismutase and glutathione peroxidase–as well as the concentrations of their cofactors–zinc, copper, and selenium–in plasma and in erythrocytes were determined. The thiobarbituric acid-reactive substances (TBARS) concentration served as an indicator of oxidative stress intensity in plasma and erythrocytes. It was found that in the control group the concentration of copper in erythrocytes was positively correlated with Km and Vmax. When the concentration of copper increased, the shape of the kinetic curve changed from sigmoidal to hyperbolic. In the control group, the concentration of zinc in erythrocytes also correlated with Km. However, the results obtained for the group of hemodialyzed patients were the opposite: when the erythrocyte concentration of copper increased, a
