Do the pTYB, pTXB, pTWIN or pCYB vectors carry any marker for the selection of the recombinant plasmids?
No. However, to reduce the background from self-ligation of the vector, the ligation sample can be digested prior to transformation with an enzyme whose site is deleted from the polylinker during cloning and is also absent in the insert. This will linearize any remaining parental vectors without the insert.
Related Questions
- How can the recombinant plasmid (pTYB, pTXB or pCYB vector with an insert) be analyzed by restriction digestion when the SapI or SmaI site is used for cloning?
- Do the pTYB, pTXB, pTWIN or pCYB vectors carry any marker for the selection of the recombinant plasmids?
- Can single-stranded DNA be prepared from the pTYB, pTXB, pTWIN and pCYB vectors?
