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Do the methylated and unmethylated DNA controls interfere with the experimental input DNA that will be immunoprecipitated?

April 26, 2017controls dna experimental immunoprecipitated input interfere methylated

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Do the methylated and unmethylated DNA controls interfere with the experimental input DNA that will be immunoprecipitated?

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No. It does not interfere with any kind of DNA except the DNA from Arabidopsis, because the Methylated DNA and Unmethylated DNA are sheared DNA from Arabidopsis thaliana. After the shearing, the corresponding DNA has been enzymatically modified with Sss I methylase in vitro in order to produce the Methylated DNA.