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Do the hydrogels change compliance over time since they have been in the cell culture medium for days?

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Do the hydrogels change compliance over time since they have been in the cell culture medium for days?

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No. The compliance is set by the amount of Extralink, the concentration of the Glycosil and Gelin-S, and the ratio of Glycosil to Gelin-S. Adding the media to the hydrogels does not change the compliance since the chemical structure of the hydrogel is fixed.

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