Do the CyDye™ DIGE Fluor minimal dyes cause suppression in MS ionization?
Generally, spot picking is performed on gels containing unlabelled samples stained with SYPRO™ Ruby. If you wish to perform MS on gels containing labelled proteins, we have no evidence that the dyes will cause suppression in MS ionization. However, the majority of proteins within the sample will remain unlabelled due to the minimal labelling approach. Therefore it is unlikely that a labelled protein will be detected by the mass spectrometry analysis.
Related Questions
- CyDye™ DIGE Fluor Cy™2, Cy3 and Cy5 minimal dyes have different quantum yields. Does this effect the accuracy with which protein abundance changes can be quantified?
- How long will my CyDye™ DIGE Fluor minimal dyes keep once I have reconstituted them?
- Do the CyDye™ DIGE Fluor minimal dyes cause suppression in MS ionization?
