Do tags interfere with antibody production?
Abbiotec does not recommend fusion proteins when producing an antibody. Large tag proteins such as GST and MBP are highly immunogenic and therefore dilute the population of antibodies made against the fused fragment. Abbiotec recommends cleaving the fusion off before immunization, especially for small fragment fused to large tags. Other tags like 6xHis, Myc, or FLAG can be used without biasing the immune response against the fused fragment.
