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Do proteins require purification prior to measurement on the NanoDrop 1000?

April 26, 2017measurement nanodrop prior Proteins purification require

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Do proteins require purification prior to measurement on the NanoDrop 1000?

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Yes when using the Protein A280 module since absorbance measurements will be affected by the presence of non-protein molecules which absorb at 280 nm. For non-purified proteins there are other pre-configured modules (BCA, Bradford, Lowry, Pierce 660 nm) that may be used.