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Do nucleic acids require purification prior to measurement on the NanoDrop 1000?

April 26, 2017acids measurement nanodrop nucleic prior purification require

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Do nucleic acids require purification prior to measurement on the NanoDrop 1000?

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Yes, absorbance measurements are not specific for a particular nucleic acid and will be affected by the presence of nucleotides and other molecules which absorb at 260 nm.