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Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?

April 26, 2017amplification Binding dna mitochondrial mutations primer repli-g target

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Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?

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No, there is no risk that mitochondrial DNA will not be amplified due to a change in the primer binding site (i.e., caused by a deletion). This is because several primers are used in the amplification…