Do LUX primers have more specificity than Taqman or other methods of detection for Real Time RT-PCR or qPCR?
LUX primers have greater specificity than SYBR green. However, compared to Taqman, LUX has the same level of specificity or in some cases slighly lower specificity than Taqman. Both of these systems have sequence specific probes, so they are specific to the gene that is being examined. However, Taqman assays require an additional target specific probe in addition to the primers. Therefore, in a few cases, a TaqMan assay may be more specific than LUX. The advantages of LUX primers over Taqman probes are that LUX is more affordable because an additional probe is not necessary and a melting curve analysis can be performed to verify specificity. Additionally, only one primer is labeled with a dye when using the LUX system.
