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Do I need to use phenol red free media to culture viruses for use in the chemiluminescence tests?

April 26, 2017chemiluminescence culture Free media phenol red tests viruses

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Do I need to use phenol red free media to culture viruses for use in the chemiluminescence tests?

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No. Viruses are diluted in assay buffer before performing the IC50 test, meaning that the phenol red is diluted enough so as not to interfere with the chemiluminescence signal.